Sanger Sequencing

Sanger Sequencing


The first method of sequencing the
genetic code was devised by Fred Sanger. To sequence the DNA, it must first
be separated into two strands. This strand to be sequenced is copied
using chemically altered basis. These altered bases cause the copying process to stop each time
one particular letter is incorporated into the growing DNA chain. This process is carried out for all four bases, and then the fragments are put together like a jigsaw
to reveal the sequence of the original piece of DNA.

49 thoughts on “Sanger Sequencing

  1. thanks this actually helps. It looks cool too, makes me concentrate more and understand for the test tomorrow even though it is very narrowed

  2. Good representation and easy to understand but still made me cringe a little when they said 'letter' in place of either base or nucelotide

  3. Agree! This is lucidity at its finest. Short, sweet, and the video clears up what words struggle to.

    Additionally, the reaction mixture contains normal A, T, C & G triphosphates as well as fluorescently-labelled letters* (to visually mark them) that terminate elongation whenever they're paired with the template sequence, which is why the strands being copied terminate at different lengths.

    *The "altered bases" mentioned, i.e. dideoxynucleotidetriphosphates (ddNTPs).

  4. Wouldn't it be awesome if professors used a video to replace each slide of powerpoint that contains the worst diagrams ever..

  5. it is soo easy… i dont understand why some teacher can't explain it, that students understand it… better leave the school and learn with youtube…

  6. iono about you guys but i always look for videos like this that are short. jesus christ took 51 secs to understand sanger fuck yea

  7. You have four tubes containing regular nucleotides in each, the first additionally containing ddATP, the second ddCTP, the third ddGTP and the fourth ddTTP.In the first tube, there are now many strands being synthesized, so, randomly, a ddATP is inserted instead of a regular dATP,causing the synthesis to stop there.This results in strands of differing length. If you run this first tube on a gel now, it will show where on the strand the A-nucleotides are.

  8. We do this in all four tubes, showing us where the As,Cs,Gs and Ts are.If we put all the ddNTPs into one tube together, the polymerase would simply insert a random ddNTP anywhere,so the resulting gel would simply tell us how many basepairs our sequence has, not what its nucleotidesequence is.

  9. I can't believe how easy it was to learn this in 53 seconds when I've been trying to understand it for 3 days! thanks!

  10. I have been trying to understand this for the past hour and a half and all it took was a 50 second video that did more for me than my overpriced college biology book. Thank You.

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